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Two distinct insulin-like growth factor 1 receptors of the mandarin fish Siniperca chuatsi (scIGF1R1 and scIGF1R2) were cloned and characterized in this study. The complementary DNA of scIGF1R1 [4254 base pairs (bp)] and scIGF1R2 (4248 bp) encoded 1418 and 1416 amino acids, respectively. Both receptors contained a single short transmembrane domain, two receptor L domains and a furin-like cysteine-rich...
One clone (Lip906) exhibiting lipase activity was screened from a metagenomic library by using a medium containing tricaprylin. A novel lipase gene from the inserted fragment of Lip906 was obtained by sequencing. The phylogenetic analysis of Lip906 lipase exhibited 34% and 32% homologue to lipases from Streptomyces sp. MspMP-M5 and Rhodopirellula europaea. This gene was expressed in Escherichia coli...
Han, S. P., X. Chen, B. Cox, C.-L. Yang, Y.-M. Wu, L. Naes and T. C. Westfall. Role of neuropeptide Y in cold stress-induced hypertension. Peptides 19(2) 351-358, 1998.-Chronic cold stress (4 o C) produced a sustained increase in mean arterial pressure in both normotensive and borderline hypertensive rats (BHR). The high blood pressure in BHRs was significantly reversed by a neuropeptide Y...
Han, S. P., X. Chen, Y. M. Wu, L. Naes and T. C. Westfall.Elevated neuropeptide Y gene expression and release during hypoglycemic stress.Peptides 18(9) 1335-1340, 1997.-Our previous studies show that neuropeptide Y (NPY) is involved in mediating sympathetic nerve stimulation-induced vasoconstriction. Insulin hypoglycemia is known to produce increased sympathetic output and elevated arterial pressure...
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