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The aim of this study was to explore the primary chemical properties and anti‐fatigue effect in vivo of Pholiota nameko polysaccharide (PNP). Through UV‐visible spectrum, the absorption peaks of proteins, nucleic acids and pigments were not found. The organic functional groups of polysaccharides (3,289.97, 1,584.72, and 1,045.23 cm–1 so on) were measured by IR spectroscopy. The PNP was a semi‐crystalline...
The sweet‐tasting protein brazzein is the smallest sweet protein with high sweet potency. Overexpression of this protein in a heterogenous host is an essential way for its production in food industry. In this study, the gene of minor form brazzein was cloned into the pET‐SUMO vector with optimized codon usage and expressed in E. coli BL21‐CodonPlus (DE3)‐RIL. The recombinant protein in absence of...
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