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Optical recording in vivo is severely interfered by heartbeat and respiratory noises. Here we tested if these noises can be removed from in vivo optical recordings from the primary auditory cortex of the guinea pig, using independent component analysis (ICA). We applied a fast ICA algorithm combined with principal component analysis to optical recordings of long durations (9-40 s). Our results show...
Optical recording in vivo severely suffers from the interference of heartbeat noise. So far, heartbeat noise has been minimized by subtracting from each experimental trial an average of interlaced control recordings. This method, however, is time-consuming and increases tissue damage due to phototoxicity. Here we applied independent component analysis (ICA) to in vivo optical recordings, for separation...
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