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A sample pretreatment method was developed for the analysis of six sex hormones in fish by subcritical 1,1,1,2-tetrafluoroethane (R134a) extraction coupled with gas chromatography-mass spectrometry(GC-MS). After the extraction process was completed, the fatty co-extracted interferent of the sample was removed by freezing filtration. Then the analytes were cleaned up by C 18 and NH 2 ...
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