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Experiments were carried out on the cryopreservation of common carp (Cyprinus carpio) sperm in order to test the suitability of using 1.2 and 5ml straws and to investigate the ploidy of malformed larvae found among the hatched progeny. In the first set of experiments, the effect of freezing time was investigated on the hatch rate of embryos. The highest hatch rate for 1.2ml straws was 69±16% at the...
In our earlier experiments on the cryopreservation of African catfish (Clarias gariepinus) sperm a high number of malformed individuals were found among the larvae hatched from eggs fertilized with frozenthawed sperm. In our present study we explored one of the possible reasons of this malformation by examining the ploidy of the larvae. Sperm was frozen in a 6% fructose extender containing 10% methanol...
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