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Twenty-eight Holstein cows (4 with ruminal cannulas) were blocked by days in milk into 7 groups and then randomly assigned to 1 of 7 balanced 4×4 Latin square diet sequences. The diets contained [dry matter (DM) basis] 20% alfalfa silage, 35% corn silage, and 45% concentrate mainly from high-moisture corn and soybean meal. Diets differed in crude protein (CP) content and source of protein supplement:...
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