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A sensitive and rapid ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to determine methadone, EDDP, fluoxetine, norfluoxetine, venlafaxine and O-desmethylvenlafaxine in rat plasma using diazepam as the internal standard (IS). Sample preparation was accomplished through a simple one-step protein precipitation with 200 µL acetonitrile added to 100 µL...
A rapid, sensitive and highly selective liquid chromatography/tandem mass spectrometry (LC–MS–MS) using an atmospheric pressure chemical ionization (APCI) method was developed to determine the concentration of trans-resveratrol in rat plasma. Samples were extracted using ethyl acetate after daidzein was added as an internal standard (IS). The main advantages of this method were the high sensitivity...
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