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Purpose. To accelerate diabetic wound healing with TGF-β1 gene delivery system using a thermosensitive hydrogel made of a triblock copolymer, PEG-PLGA-PEG. Methods. Two 7 × 7 mm full thickness excisional wounds were created in parallel at the back of each genetically diabetic mouse. The hydrogel containing plasmid TGF-β1 was administered to the wound and formed an adhesive film in situ. Controls...
Purpose. To evaluate the biologic effect of direct cutaneous TGF-β1 gene delivery on impaired wound healing models using genetically diabetic mice. Methods. Diabetic mice (C57BKS.Cg-m +/+ Leprdb female mice) with 1 cm × 1 cm excisional wounds were intradermally injected with 60 μg of plasmid DNA encoding TGF-β1 gene. The wound closure was measured up to 14 days postwounding. At days 7 and 14...
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