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The novel gene Mipu1 was recently identified in rat due to its up-regulation in response to myocardial ischemia preconditioning. We previously demonstrated that Mipu1 was a nuclear protein and a transcriptional repressor. In this study, Mipu1 was expressed in E. coli and purified using a recombinant expression system and a purification protocol. Milligram quantities of highly purified Mipu1 were obtained...
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