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Adenylate cyclase‐hemolysin toxin (CyaA) produced from the human respiratory tract pathogen Bordetella pertussis requires fatty‐acyl modification by CyaC‐acyltransferase to become an active toxin. Previously, the recombinant CyaA pore‐forming (CyaA‐PF) fragment expressed in Escherichia coli was shown to be hemolytically active upon palmitoylation in vivo by cosynthesized CyaC. Here, the 21‐kDa CyaC...
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