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Deterioration in the quality of mammalian mature oocytes during metaphase-II (M-II) arrest is called “oocyte aging”. Although histone acetylation may affect the progression of aging in murine oocytes, the mechanism is unknown. The objective was to determine the role of ooplasmic reactive oxygen species (ROS) in acetylation of histone H4 at lysine 12 (acH4K12) in porcine aged oocytes in vitro. Based...
The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is widely used as a eukaryotic expression vector for protein production. In the current study, chicken β-globin 5′-HS4 insulator (HS4) was placed downstream of the polyhedrin promoter-directed foreign gene expression cassette in AcMNPV, and found to markedly increase the expression of target gene. When enhanced green fluorescence...
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