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The melibiose carrier from Escherichia coli is a galactoside-cation symporter. Based on both experimental evidence and hydropathy analysis, 12 transmembrane helices have been assigned to this integral membrane protein. Transmembrane helix 2 contains several charged and polar amino acids that have been shown to be essential for the cation-coupled transport of melibiose. Starting with the cysteine-less...
Systems for overexpression and purification of active α, β and γ subunits of Escherichia coli H + -ATPase were established. The α and β subunits recovered as soluble form were purified by hydroxyapatite column chromatography. Since the γ subunit was overexpressed as the insoluble form, this subunit was purified by polyacrylamide gel-electrophoresis containing sodium dodecyl sulfate. By...
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