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Many high-throughput DNA sequencing methods provide very short reads, typically of a few tens of base pairs. New algorithms and softwares are needed to analysis those short reads effectively. There are some software tools for aligning short reads to a reference genome. Here we introduce a new method, Umap, to align theses short reads faster and more exact. Applying Umap to simulated very short reads...
To mimic natural extracellular matrix architecture, the poly (L-lactic acid) (PLLA) scaffolds loading icariin and icariin-2-hydroxypropyl-beta-cyclodextrin inclusion complex were prepared via phase separation, solvent replacement and freeze-drying. The morphologies of PLLA scaffolds were visualized with scanning electron microscopy, the icariin concentration and drug release in vitro were determined...
This study was aiming to investigate the fabrication and characterization of the porous chitosan/gelatin scaffold cross-linked with genipin (GP) which is widely used as a natural and non-toxic cross-linking agent. The chitosan/gelatin scaffold was prepared and cross-linked with 1% or 2%(w/w) genipin for 24 hours at 37degC, then pre-freezing for 48h at -20degC and -80degC before freeze drying. As a...
Biodegradable polymers have been widely used as scaffolding materials to regenerate new tissues. To mimic natural extracellular matrix architecture, a novel highly porous structure, which is a three-dimensional interconnected fibrous network with a fiber diameter ranging from 100 to 500 nm, has been created from poly (L-lactic acid) in this work. These fibrous matrices were prepared from the polymer...
In the present study, the functions of bovine chondrocytes on tetrandrine-loading poly (L-lactic acid) (PLLA) films were investigated in vitro. The surface characteristics of control PLLA and tetrandrine-loading PLLA films were examined by water contact angle measurement and electron spectroscopy for chemical analysis (ESCA). The contact angle indicated the change in hydrophilicity and the ESCA data...
The objective of this study was to investigate the efficiency of poly (L-lactic acid) (PLLA) surface modification with chitosan and gelatin. The properties of original PLLA films and modified PLLA films were investigated by water contact angle measurement and electron spectroscopy for chemical analysis (ESCA). The attachment and proliferation of bovine articular chondrocytes seeded on modified PLLA...
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