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Non-alcoholic fatty liver disease (NAFLD) is a kind of prevalence diseases. Traditional Chinese medicine (TCM) has better efficacy on treating NAFLD. But there are also not known about the critical pathogenesis and the corresponding biological factors. Regarding this, we addressed a text mining approach to analyze the pattern profile, rule of medication, and the pathological factors of NAFLD from...
The pulA gene enconding a thermostable pullulanase of 844 amino acids was cloned by PCR amplification from the genomic DNA of Thermotoga maritima MSB8. We successfully expressed the pulA gene in a protease-deficient strain Bacillus subtilis WB600, and the expressed pullulanase was secreted into the culture broth. Preliminary results showed that the pullulanase exhibited optimal activity at 90°C, and...
Wastewater treatment practices have been significantly improved since the introduction of novel microbial treatment technologies. The Sequencing Batch Reactor (SBR) system for ammonia removal over nitrite (SHARON) process by intermittent aeration as well as the key parameters of SHARON, were studied in this paper, which includes pH, temperature, dissolved oxygen (DO) and sludge age, to obtain the...
Pectate lyase obtained the wide-spread application in the Chinese traditional medicine extraction because it can remarkably reduce impurity content and viscosity of the plant materials. However the low enzyme activity and monoicous species hindered the application of enzymatic method to the extraction process of the herbal medicine. In this paper, we constructed a engineering strain which can overexpressed...
Three recombinant Escherichia coli strains were constructed to produce guanosine 5'-diphosphate (GDP)-mannose, donor of GDP-fucose, which is an essential substrate for synthesis of fucosyloligosaccharides. Glucokinase (glk), phosphomannomutase (manB), and mannose-1-phosphate guanylytransferase (manC), are three crucial enzymes for the de novo GDP-mannose biosynthesis, were overexpressed in recombinant...
Arthrobacter simplex is extensively used in pharmaceutical industry to introduce C1-C2 double bond into steroid nucleus. This dehydrogenation reaction is known characterized by its 3-ketosteroid-Δ1-dehydrogenase(KSDH), which is encoded by ksdD gene. In this research, we report the expression of KSDH in Escherichia coli. The activity of the enzyme has been determined by spectrophotometric method. We...
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