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A method is described for covalent immobilization of uricase onto polyethylene terephthalate (PET) membrane with a conjugation yield of 4.44μg/cm 2 and 66.6% retention of initial activity of free enzyme. The enzyme exhibited an increase in optimum pH from pH 7.0 to 8.5 and K m for uric acid from 0.075mM to 0.13mM but slight decrease in temp. for maximum activity from 37°C to 35°C after...
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