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Salt crystallizing agents studied to date generally induce a protein salting-out phenomenon at high concentrations, whereas the addition of NiCl 2 over 0.5M induces an increase in the solubility of the tetragonal form crystals of hen egg-white lysozyme. To elucidate this particular effect of NiCl 2 on the solubility, the structures of the crystals grown at pH 4.7 in the presence of...
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