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Assays for non-enzyme protein based on peptide–protein interaction are few due to the fact that most of peptide–protein bindings do not produce easily measurable output signals. Here we report a homogenous assay for colorimetric and quantitative detection of a cancer marker and promising antitumor target, cyclin A 2 , using noncrosslinking aggregation of unmodified AuNPs/AgNPs by utilizing...
A label-free colorimetric assay, using non-crosslinking AuNP aggregation, has been developed for the screening of specific triplex DNA binders. The relative binding affinities can be simultaneously determined. Our novel assay is simple in design and fast in operation, avoiding either AuNPs modification or oligonucleotide labeling, and easy to implement for visual detection. This strategy may offer...
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