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BACKGROUNDExtracellular polymeric substances (EPSs) play an essential role in the performance of biological wastewater treatment. It is necessary to understand the correlation between EPSs and the carbon sources and sludge‐retention time (SRT) of the activated‐sludge process to gain efficient operational strategies.
RESULTSPolysaccharides (PSs) and proteins (PNs) were the main constituents in EPSs. The percentage of PSs and PNs in the loosely bound (LB‐) EPSs and tightly bound (TB‐) EPSs were 18–90% and 18–85%, respectively. The PS/PN ratios in the LB‐EPS and TB‐EPS were in the range 0.38–2.50 and 0.30–2.34, respectively. The contents of LB‐EPSs and TB‐EPSs were both slightly higher in sludge samples cultivated by starch than by those cultivated by glucose. Compared with the sludge samples grown in glucose, the PSs content of the LB‐EPS was higher than in other samples, whereas the PNs content was lower in the LB‐EPS from sludge flocs grown in starch than those grown in glucose. PSs yield of TB‐EPSs was almost equal in the two carbon sources, whereas PNs yield was slightly higher in TB‐EPSs from samples cultured with glucose than with starch...
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