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Background: Many variations in avian in ovo transfection of the neural tube/crest have been reported, but never compared quantitatively. Results: Genome integrating pT2K‐CAGGS‐GFP and pCAGGS‐T2TP transposase plasmids were co‐electroporated into quail E2 embryo trunk neural tube and the proportion of GFP‐expressing neural cells was counted 1 and 7 days later. Electroporation efficiency increased with...
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