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Chitosan-thiobutylamidine was developed and evaluated as a novel tool for gene delivery. The conjugate, displaying 299.1±11.5μmol free thiol groups per gram polymer, formed coacervates with pDNA at a mean size of 125nm and a zeta potential of +9mV. Thiol groups, being susceptible for oxidation, were immobilised on the polymeric backbone of chitosan in order to introduce the property of extracellular...
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