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We designed and synthesized a novel probe Cou-Rho-SA-Cu (II) for detection of thiols based on a coumarin-rhodamine FRET system. The compound Cou-Rho-SA exhibited strong blue fluorescence as the rhodamine moiety of the compound Cou-Rho-SA was in a ring-closing form and no FRET process occurred. The probe Cou-Rho-SA-Cu (II) was prepared by addition of Cu2+ to the solution of Cou-Rho-SA. In the absence...
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