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The senescence-associated β-galactosidase (SA-βG) assay is one of the few accepted markers of cell aging. However, the cytochemical method using 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-Gal) as substrate is limited in sensitivity and is only semiquantitative. Here, we modified the X-Gal method by replacing X-Gal with fluorescein di-β-d-galactopyranoside (FDG) as substrate for SA-βG, and...
Cellular ATP is commonly determined as production of bioluminescence using a luciferin–luciferase reaction system. Before the measurement of bioluminescence, cellular ATP must first be extracted. Two commonly used extraction methods are: (1) Tris–borate buffer (pH 9.2) coupled with a heating process (to inactivate ATPase) and (2) perchloric acid followed by neutralization. However, we found that both...
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