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Compared with traditional pathogenic producers, Bacillus subtilis as a Class I microorganism offers many advantages for industrial‐scale 2,3‐butanediol production. Unlike previous reports in which two stereoisomers (with a ratio of 3:2) were produced, we first found that wild type B. subtilis 168 generates only D‐(−)‐2,3‐butanediol (purity >99%) under low oxygen conditions. The total high yield...
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