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Purpose: To evaluate human tear fluid for transforming growth factor beta isoforms 1 and 2 (TGF-β1 and TGF-β2). Methods: To accomplish this, human tears were evaluated for TGF-βs by quantitative antibody sandwich ELISA (sELISA), mink lung epithelial cell (MLEC) growth inhibition bioassay and western blotting. Various physical and chemical treatments were used to activate TGF-β in these assays. ...
Purpose: Transforming growth factor beta (TGF-β) isoforms 1 and 2 have recently been detected in stimulated human tear fluid. The purpose of this study was to determine if these TGF-βs are produced and secreted by the lacrimal gland. Methods: To accomplish this, reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect expression of TGF-β1 and TGF-β2 mRNAs in normal human and rabbit...
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