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Various research models have been developed to study the biology of disc cells. Recently, the adult disc nucleus pulposus (NP) has been well studied. However, the immature NP is underinvestigated due to a lack of a suitable model. This study aimed to establish an organ culture of immature porcine disc by optimizing culture conditions and using a self‐developed substance exchanger‐based bioreactor...
Osmolarity fluctuations are inevitable within the nucleus pulposus (NP). However, the effects of osmolarity on NP cell apoptosis within the organ‐cultured disc remain unclear. The objective of this study was to investigate effects of different osmolarity levels (hypo‐, iso‐, and hyper‐) and osmolarity modes (constant and cyclic) on NP cell apoptosis in a disc perfusion culture and to study the role...
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