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ω-Grammotoxin SIA (GrTx) is a 36 amino acid residue protein toxin from spider venom that inhibits P/Q and N-type voltage-gated Ca 2+ channels by modifying voltage-dependent gating. We determined the three-dimensional structure of GrTx using NMR spectroscopy. The toxin adopts an ''inhibitor cystine knot'' motif composed of two β-strands (Leu19-Cys21 and Cys30-Trp32) and a β-bulge (Trp6,...
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