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A Pt layer/Pt disk electrode configuration was used as a scanning electrochemical microscopy (SECM) probe. The glass seal part of the insulator was covered with a Pt layer to form an exposed pseudo reference electrode. In a HEPES-based medium at pH 7.5, the half-wave potential (E 1/2 ) for [Fe(CN) 6 ] 4− oxidation and O 2 reduction measured versus the internal Pt pseudo...
Single-cell analysis has become a powerful method to construct a new type of whole cell sensor integrating with individual cellular responses all together. However, sensitivity of electrochemical detection system in the present stage is insufficient to discuss in detail about the results obtained from a large number of data set because the individual single-cell responses are largely fluctuated. To...
Electrochemical assay using HeLa cell lines transfected with various plasmid vectors encoding SEAP (secreted alkaline phosphatase) as the reporter has been performed by using SECM (scanning electrochemical microscopy). The plasmid vector contains different responsive elements that include GRE (glucocorticoid response elements), CRE (cAMP responsive elements), or κB (binding site for NFκB (nuclear...
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