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The nucleotide (nt) sequence of the 3 end of the yeast HIS3 mRNA was determined by PCR amplification of the 3 end. Analysis of 28 individual clones revealed that at least 13 distinct polyadenylation sites are present. The sites of polyadenylation are extremely heterogeneous and do not show any obvious similarity other than that they occur after pyrimidine residues in most cases. Most mutants...
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