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The effects of eight microelements (I−, BO33−, MoO42−, Co2+, Cu2+, Mn2+, Fe2+, Zn2+) on the biosynthesis of camptothecin and the growth of suspension cultures of Camptotheca acuminata were studied. The increase of I− to 25 μM l−1, Cu2+ to 1 μM l−1, Co2+ to 2 μM l−1 and MoO42− to 10 μM l−1 in Murashige and Skoog (MS) medium resulted in 1.66, 2.84, 2.53 and 2.04 times...
Nitrate at 70 m gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4+/NO3− molar ratio of 5:1 (giving a total of 40 m N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280% and 340%, respectively when compared with...
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