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The pattern of genome transcription in prokaryotes is determined by selective distribution of RNA polymerase within the genome. The gene selectivity of RNA polymerase is modulated after interaction with DNA-binding transcription factors (TFs). Escherichia coli contains a total of about 300 TFs, but the regulatory function has not yet been identified for about one third. For quick and systematic search...
Under stressful conditions in nature, Escherichia coli forms biofilms for long-term survival. Curli fimbriae are an essential architecture for cell-cell contacts within biofilms. Structural components and assembly factors of curli are encoded by two divergently arranged operons, csgBA and csgDEFG, which are regulated by the csgD gene product. Thus, CsgD is a master regulator of bacterial biofilm formation...
Escherichia coli adapts to changes in external environment by selective transcription of a total of about 4,400 genes on its genome. In each case of the adaptation, the expression of a specific set of stress-response genes is controlled mainly at the step of transcription initiation by selective recognition of the promoters by the RNA polymerase. The RNA polymerase core enzyme binds to one of seven...
Escherichia coli carries a sophisticated genetic system for adaptation to changes in external environment by selective transcription of a total of about 4,400 genes on its genome. Gene selection takes place at the step of promoter recognition by the RNA polymerase. The promoter selectivity of RNA polymerase is, however, modulated after interaction with DNA-binding transcription factors. In E. coli,...
The authors devised a 3-D display with a linearly moving mirror to reflect a series of 2-D cross sections of the 3-D image on a CRT. The crystallography data in a protein data bank can be instantaneously converted into real 3-D images. Structures of several proteins and t-RNAs are presented as examples.<<ETX>>
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