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The method of high-performance liquid chromatography (HPLC) with UV–vis detection was used and validated for the simultaneous determination of six flavonoids (puerarin, rutin, morin, luteolin, quercetin, kaempferol) and troxerutin in rat urine and chicken plasma. Chromatographic separation was performed using a VP-ODS column (150mm×4.6mm, 5.0μm) maintained at 35.0°C. The mobile phase was a mixture...
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