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Eisenia andrei, Lumbricus rubellus and Lumbricus terrestris were exposed to 250, 250 and 350mgkg −1 Cu respectively in Cu(NO 3 ) 2(aq) amended soil for 28 d. Earthworms were then depurated for 24 to 72h, digested and analysed for Cu and Ti or, subsequent to depuration were dissected to remove any remaining soil particles from the alimentary canal and then digested and analysed...
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