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A sensitive pre-column derivatization method was developed for analysis of carbohydrates by HPLC with fluorescence detection. The introduction of 2-(12-benzo[b]acridin-5(12H)-yl)-acetohydrazide (BAAH) with excellent fluorescence property into the molecules of monosaccharides greatly enhanced the HPLC sensitivity of the analytes. Meanwhile, derivatization with BAAH also greatly increased the hydrophobicity...
A new labeling reagent for fatty acids, 1-(9H-carbazol-9-yl) propan-2-yl-methanesulfonate (CPMS), has been synthesized and successfully applied to the HPLC determination of fatty acids in traditional Chinese herb Notopterygium forbesii Boiss. The reaction of CPMS with fatty acids could proceed easily and quickly in the presence of K 2 CO 3 catalyst within 30min. The derivatives exhibit...
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