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Snakes are very unique animals that have distinguished motor function adaptable to the most diverse environments in terrestrial animals regardless of their simple cord-shaped body. Revealing the mechanism underlying this distinct locomotion pattern, which is fundamentally different from walking, is significant not only in biological field but also for applications in engineering field. However, it...
We investigated the interaction of large DNA with protamine, a small basic protein, using fluorescence microscopy and electron microscopy. In most vertebrate sperm cells, a large DNA is packaged by protamine into a highly compact, biologically inactive form of chromatin. The manner of packaging plays a key role in spermatogenesis. We found that, with increase of protamine concentration, DNA molecules...
Methods for turning on and off gene expression at the experimentalist's discretion would be useful for analyzing various biological processes. They would also serve as valuable tools for manipulating biological processes in living organisms. We have been developing a novel microscopic system utilizing an infraredinfrared laser laser, IR-LEGO (infrared-laser evoked gene operator), which is designed...
Ectopic expression via transgene is powerful means for functional analysis of genes of multicellular organisms in vivo. Use of tissue-specific promoters for ectopic expression is limited by their inherent properties to drive gene expression in a restricted temporal-spatial expression pattern. We have developed a novel microscopic system utilizing an infrared laser, IR-LEGO (infrared-laser evoked gene...
We have been developing multi-scale affinity sensor which can be applied from 10 μm (single cell) to 1 mm ranges based on two-dimensional surface plasmon resonance (2D-SPR) imaging. But SPR sensitivity was drastically decreased in 10 μm wells because surface plasmon wave cannot propagate in such micro area. The use of shorter wavelength incident light might improve sensitivity of SPR sensing in micro...
In this paper, a versatile, rapid and reproducible method for patterning different cell types based on negative dielectrophoresis (n-DEP), without any special pretreatment of a culture slide, has been described. An interdigitated array (IDA) electrode with four independent microelectrode subunits was fabricated with indium-tin-oxide (ITO) and used as a template to form cellular micropatterns. A suspension...
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