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Plutella xylostella (PX) that were 130000-fold more resistant to Cry1Ac were selected from the susceptible strain and maintained in the laboratory. The LC 50 of the susceptible strain (PXS) was 0.38 μg toxin/g diet, whereas that of the resistant strain (PXR) was 4800 μg toxin/g diet. Brush border membrane vesicles (BBMV) were prepared from both PXS and PXR. In ligand blot analysis, Cry1Ac...
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