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Biocatalytic reduction of methyl o-chlorobenzoylformate (CBFM) provides a green and direct access to methyl (R)-o-chloromandelate [(R)-CMM], an intermediate for a platelet aggregation inhibitor named clopidogrel. As much as 500gL −1 of CBFM was stoichiometrically converted into enantiopure (R)-CMM at 20°C by using a whole-cell catalyst coexpressing an aldo-keto reductase from Bacillus sp....
A system for the one-pot synthesis of diosgenyl-β-d-glucopyranoside (trillin) using multiple recombinant enzymes is developed. The enzymes maltodextrin phosphorylase (E1), glucose-1-phosphate thymidylyltransferase (E2), inorganic pyrophosphatase (E3), and solanidine glucosyltransferase (E4) involved in the work have been cloned and expressed in Escherichia coli. Under the optimized reaction conditions,...
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