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•Subcutaneous inoculation of ZIKV causes fever and viremia in NHPs•Robust viral RNA excretion was seen in urine, saliva, and lacrimal fluid.•The intestinal tracts, spleen, parotid glands, and lymph nodes us are potential target organs.•ZIKV infection induces robust humoral and cellular immune response.Zika virus (ZIKV) is spreading throughout the world whereas no vaccine or drug is currently available...
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