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A cDNA encoding 25-hydroxyvitamin D 3 24-hydroxylase (P450cc24) was isolated from a rat kidney cDNA library using specific antibodies to the enzyme. The isolated cDNA wax 3.2 kbp long and contained a 1542-bp open reading frame encoding 514 amino acids. The deduced amino acid sequence contained a presequence typical or mitochondrial enzymes in the N-terminal region. The amino acid sequence...
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