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l-selectin mediates the initial tethering and rolling of lymphocytes in high endothelial venules. To study the transcriptional regulation of mouse l-selectin, we cloned 4.5 kb 5′-flanking sequences of the mouse sell. Luciferase analysis of serial 5′-deletion mutants showed that the first 285 bp was sufficient to drive high promoter activity in EL4 cells, but not in Sell-negative HeLa cells, suggesting...
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