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Registration of an in vivo microscopy image sequence is necessary in many significant studies, including studies of atherosclerosis in large arteries and the heart. Significant cardiac and respiratory motion of the living subject, occasional spells of focal plane changes, drift in the field of view, and long image sequences are the principal roadblocks. The first step in such a registration process...
Discovery is ongoing regarding the causes and onset of atherosclerosis. Studying the movement of dendritic cells may provide insight to disease pathways and mechanisms, which will eventually lead to improved treatment. This paper develops an approach, inspired by momentum, to quantify the amount of quivering motion separately from translational motion. A simulation is developed to contrast the method...
Registration of in vivo microscopy image sequences is important for tracking of cells. Registering a long sequence of in vivo microscopy images is particularly challenging for several reasons, which include motion artifacts created by the cardiac cycle and breathing movements of the living subject, occasional defocussing, illumination change, and noise in image acquisition. To accommodate these variations,...
Recent advances in multiphoton microscopy have allowed the capture of dendritic and T cells on video in a 3D volume. This paper reports on an approach for automatically detecting and tracking the cells and collecting statistics on their characteristics of motion and interaction durations. A novel method to extend the track longevity is presented, where an adaptive acceptance gate is computed based...
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