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Association between multimorbidity and postoperative mortality in patients undergoing major surgery: a prospective study in 29 countries across Europe

STARSurg Collaborative, EuroSurg Collaborative, S. K. Kamarajah, O. Kouli, więcej

Background Multimorbidity poses a global challenge to healthcare delivery. This study aimed to describe the prevalence of multimorbidity, common disease combinations and outcomes in a contemporary cohort of patients undergoing major abdominal surgery. Methods This was a pre‐planned analysis of a prospective, multicentre, international study investigating cardiovascular complications after major...

artykuł

Harnessing metabolic plasticity in CHO cells for enhanced perfusion cultivation

Matthias Nöbel, Craig Barry, Michael A. MacDonald, Kym Baker, więcej

Biotechnology and Bioengineering > 121 > 4 > 1370 - 1382

Chinese Hamster Ovary (CHO) cells have rapidly become a cornerstone in biopharmaceutical production. Recently, a reinvigoration of perfusion culture mode in CHO cell cultivation has been observed. However, most cell lines currently in use have been engineered and adapted for fed‐batch culture methods, and may not perform optimally under perfusion conditions. To improve the cell's resilience and viability...

artykuł

Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells

Verónica S. Martínez, Karen Rodriguez, Timothy McCubbin, Junjie Tong, więcej

Biotechnology Journal > 19 > 2 > n/a - n/a

Chinese hamster ovary (CHO) cells are widely used to produce complex biopharmaceuticals. Improving their productivity is necessary to fulfill the growing demand for such products. One way to enhance productivity is by cultivating cells at high densities, but inhibitory by‐products, such as metabolite derivatives from amino acid degradation, can hinder achieving high cell densities. This research examines...

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Oviposition substrate location by the invasive woodwasp Sirex noctilio: the combined effect of chemical cues emitted by its obligate symbiont Amylostereum areolatum and different host‐tree species

Santiago Masagué, Patricia C. Fernández, Francisco Devescovi, Diego F. Segura, więcej

Pest Management Science > 79 > 10 > 3959 - 3969

BACKGROUND Sirex noctilio is an invasive forest wasp that affects pines and can result in severe economic losses. The use of semiochemicals offers an opportunity to develop sensitive and specific capturing systems to mitigatenegative impacts. Previous research showed that female S. noctilio would use volatiles emitted by its fungal symbiont, Amylostereum areolatum, but little is known about how these...

artykuł

Honeydew production by the giant willow aphid (Tuberolachnus salignus, Hemiptera: Aphididae) and its effect on foraging yellowjackets (Hymenoptera: Vespidae)

Andrés S. Martínez, José M. Villacide, Micaela Buteler, María Noel Serra, więcej

Pest Management Science > 79 > 8 > 2912 - 2919

BACKGROUND Understanding the factors that promote the success of invasive species is important for managing biological invasions. Interactions between invasives and other species (e.g. competitors, pathogens, or predators), could favor or limit their success. In recent decades, yellowjacket wasps, including Vespula germanica and Vespula vulgaris, have successfully established in Patagonia. Additionally,...

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Emerging adults’ judgments about helping at home: Situational and experiential sources of variability

Marie Grace S. Martinez, Audun Dahl

Social Development > 32 > 2 > 704 - 721

Judgments and decisions about household helping affect how families function, and they develop through everyday experiences. Much prior research has examined stable individual differences in prosocial orientations, but such orientations also vary within individuals. The present work examined situational and experiential sources of variability in emerging adults’ judgments and reasoning about helping...

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Processing Vat Polymerized Triply Periodic Minimal Surface Scaffolds of Hydroxyapatite

Cathleen A. Hoel, Sara K. Peterson, Rachel Rose, Eric Telfeyan, więcej

Advanced Engineering Materials > 25 > 1 > n/a - n/a

Vat polymerization of ceramic slurries offers a means for producing triply periodic minimal surface (TPMS) structures, which are candidates for artificial trabecular bone replacement therapy. However, structures with relevant pore sizes below 500 μm and porosities above 80% push the limits of current ceramic additive manufacturing capabilities. Herein, printing and postprocessing of TPMS structures...

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In vitro antifungal susceptibility of Fusarium species and Aspergillus fumigatus cultured from eleven horses with fungal keratitis

Paoul S. Martinez, R. David Whitley, Caryn E. Plummer, Rebecca L. Richardson, więcej

Veterinary Ophthalmology > 25 > 5 > 376 - 384

Purpose To examine the relationship between Minimum Inhibitory Concentration (MICs) and response to therapy of 6 Fusarium spp. and 5 Aspergillus fumigatus isolated from equine ulcerative keratitis cases. Procedure Fungi were identified by morphology and Internal Transcribed Spacer (ITS) polymerase chain reaction (PCR) with sequencing and evaluated at the University of Texas Fungal Testing Laboratory...

artykuł

Temperature and host plant species affect the performance and immunocompetence of an outbreak defoliator in northwestern Patagonia

María Noel Serra, Carolina Quintero, Mariano A. Rodríguez‐Cabal, Andrés S. Martínez, więcej

Ecological Entomology > 47 > 4 > 580 - 589

Rising temperature has been associated with increased occurrence of herbivorous insect outbreaks, explained by several direct and indirect mechanisms. Whereas natural enemies are known key drivers of forest‐defoliating insect cycles, indirect effects of temperature on insect's ability to defend against pathogens and parasitoids (e.g., immunocompetence), as well as the interaction with other mechanisms...

artykuł

Modeling apoptosis resistance in CHO cells with CRISPR‐mediated knockouts of Bak1, Bax, and Bok

Michael A. MacDonald, Craig Barry, Teddy Groves, Verónica S. Martínez, więcej

Biotechnology and Bioengineering > 119 > 6 > 1380 - 1391

Chinese hamster ovary (CHO) cells are the primary platform for the production of biopharmaceuticals. To increase yields, many CHO cell lines have been genetically engineered to resist cell death. However, the kinetics that governs cell fate in bioreactors are confounded by many variables associated with batch processes. Here, we used CRISPR‐Cas9 to create combinatorial knockouts of the three known...

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Are invasive species promoting yellowjacket invasion in Patagonia?

Maité Masciocchi, José M. Villacide, Micaela Buteler, Andrés S. Martínez

Journal of Applied Entomology > 146 > 5 > 547 - 556

In recent decades, yellowjacket wasps, including Vespula vulgaris, have successfully been established in Patagonia. Additionally, the invasive willow Salix fragilis has also invaded areas next to watercourses, which in turn are typically colonized by the giant willow aphid (GWA, Tuberolagnus salignus), another invasive species. The honeydew exuded by the aphids has been reported to be used as a carbohydrate...

artykuł

Ultra‐Sharp Nanowire Arrays Natively Permeate, Record, and Stimulate Intracellular Activity in Neuronal and Cardiac Networks

Ren Liu, Jihwan Lee, Youngbin Tchoe, Deborah Pre, więcej

Advanced Functional Materials > 32 > 8 > n/a - n/a

Intracellular access with high spatiotemporal resolution can enhance the understanding of how neurons or cardiomyocytes regulate and orchestrate network activity and how this activity can be affected with pharmacology or other interventional modalities. Nanoscale devices often employ electroporation to transiently permeate the cell membrane and record intracellular potentials, which tend to decrease...

artykuł

Eligible neonatal donors after circulatory determination of death (Maastricht type III): A national survey of level III NICUs

Sonia Caserío, Juan Arnaez, the Neo‐Spain Donation Group, R Izquierdo, więcej

Pediatric Transplantation > 26 > 1 > n/a - n/a

Background Organ donation continues to increase worldwide, but in general paediatric patients remain less likely to receive a transplant. The inclusion of neonates as donors after cDCD should be considered in an effort to increase donation rates. Methods The survey for a cross‐sectional national study of potential cDCD neonatal donors (Maastricht type III) was sent to all 90 level III Spanish neonatal...

artykuł

An international assessment of the adoption of enhanced recovery after surgery (ERAS®) principles across colorectal units in 2019–2020

ESCP Enhanced Recovery Collaborating Group, Thomas Pinkney, H Taylor, C Tong, więcej

Colorectal Disease > 23 > 11 > 2980 - 2987

Aim The Enhanced Recovery After Surgery (ERAS®) Society guidelines aim to standardize perioperative care in colorectal surgery via 25 principles. We aimed to assess the variation in uptake of these principles across an international network of colorectal units. Method An online survey was circulated amongst European Society of Coloproctology members in 2019–2020. For each ERAS principle, respondents...

artykuł

Physical education teaching for saving energy in basketball sports athletics using Hidden Markov and Motion Model

Ning Zhang, Yubin Han, Rubén G. Crespo, Oscar S. Martínez

Computational Intelligence > 37 > 3 > 1125 - 1140

A new trend of schooling is characterized by long‐term learning and driven by technological, social, and cultural developments. This trend means that physical education (PE) and sports science must be strengthened. Although PE and sports are practical activities, specialists can make use of modern teaching technologies. Basketball is intended to develop the skills and understanding of movement and...

artykuł

Vitamin retention during the canning of swordfish (Xiphias gladius) with different filling media

N. Cobas, L. Piñeiro‐Lago, L. Gómez‐Limia, I. Franco, więcej

Journal of Food Science > 86 > 5 > 1704 - 1713

Abstract Swordfish (Xiphias gladius) is an economically and nutritionally important fish. The aims of this study were to determine the effects of the various steps of the canning process and of different filling media (olive oil, corn oil, sunflower oil, and high oleic sunflower oil) on the fat‐ and water‐soluble vitamin contents of swordfish. An HPLC‐based method was used for this purpose. The canning...

artykuł

Minor hallucinations reflect early gray matter loss and predict subjective cognitive decline in Parkinson's disease

H. Bejr‐kasem, F. Sampedro, J. Marín‐Lahoz, S. Martínez‐Horta, więcej

European Journal of Neurology > 28 > 2 > 438 - 447

Background and purpose Well‐structured hallucinations in Parkinson’s disease (PD) are associated with poor prognosis and dementia. However, the predictive value of minor psychotic phenomena in cognitive deterioration is not well known. Cross‐sectional studies have shown that PD patients with minor hallucinations have more severe cortical atrophy than non‐hallucinators, but baseline and longitudinal...

artykuł

‘Omics driven discoveries of gene targets for apoptosis attenuation in CHO cells

Camila A. Orellana, Verónica S. Martínez, Michael A. MacDonald, Matthew N. Henry, więcej

Biotechnology and Bioengineering > 118 > 1 > 481 - 490

Chinese hamster ovary (CHO) cells are widely used in biopharmaceutical production. Improvements to cell lines and bioprocesses are constantly being explored. One of the major limitations of CHO cell culture is that the cells undergo apoptosis, leading to rapid cell death, which impedes reaching high recombinant protein titres. While several genetic engineering strategies have been successfully employed...

artykuł

A novel class of fast‐acting antimalarial agents: Substituted 15‐membered azalides

Mihaela Peric, Dijana Pešić, Sulejman Alihodžić, Andrea Fajdetić, więcej

British Journal of Pharmacology > 178 > 2 > 363 - 377

Background and Purpose Efficacy of current antimalarial treatments is declining as a result of increasing antimalarial drug resistance, so new and potent antimalarial drugs are urgently needed. Azithromycin, an azalide antibiotic, was found useful in malaria therapy, but its efficacy in humans is low. Experimental Approach Four compounds belonging to structurally different azalide classes were...

artykuł

Rethinking Hospital‐Associated Disability for Patients With COVID‐19

Maylyn S Martinez, Marla R Robinson, Vineet M Arora

Journal of Hospital Medicine > 15 > 12 > 757 - 759

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