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The measured height of DNA molecules adsorbed on a mica substrate by scanning probe microscopy is always less than the theoretical diameter. In this paper we show that, when imaged in ambient conditions, the molecules are usually immersed in the salt layer used to adsorb them to the substrate. This layer distorts the measurement of DNA height and is the main source of error but not the only one. We...
In this work, we introduce jumping mode (JM) scanning force microscopy (SFM) as a suitable technique for imaging soft samples in liquid environment like DNA adsorbed on mica. JM reveals as a non-intrusive technique where shear forces are minimized by performing the scanning motion without tip-sample contact. We find no visible damage on DNA samples and the nominal height of 2nm of the molecules is...
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