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Nucleosome positioning and stability affect gene regulation in eukaryotic chromatin. Histone H2A.Z is an evolutionally conserved histone variant that forms mobile and unstable nucleosomes in vivo and in vitro. In the present study, we reconstituted nucleosomes containing human H2A.Z.1 mutants, in which the N‐terminal or C‐terminal half of H2A.Z.1 was replaced by the corresponding canonical H2A region...
Peroxisome proliferator‐activated receptor α (PPARα) is a member of the nuclear receptor family, regulating fatty acid degradation in many organs. Two‐dimensional SDS‐PAGE of brown adipose tissue (BAT) from PPARα‐null mice produced a higher‐density spot. Proteomic analysis indicated that the protein was pyruvate dehydrogenase β (PDHβ). To observe PDHβ regulation in BAT, the organ was stimulated by...
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