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This work is focussed on the evaluation of enzymatic synthesis of galactooligosaccharides (GOS) catalysed by β-galactosidase from permeabilized cell of Kluyveromyces marxianus in different compressed fluids. The experiments were performed in a batch mode reactor, where the effects of enzyme and lactose concentrations and system pressure were evaluated. Results showed significant differences in the...
The present work investigates, as a first step, the influences of pressure, exposure time and depressurisation rate on the β-galactosidase activity of permeabilized cells of Kluyveromyces marxianus CCT 7082 submitted to treatment with compressed carbon dioxide, propane and n-butane. In general, results showed that the enzyme activity changes significantly, depending on the experimental conditions...
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