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This paper investigated a new detection method of oxytetracycline (OTC) in aquatic products with ultrasensitive detection limit. The method was constructed on the basis of raman hot spot between gold nanoparticles (AuNPs) (13nm and 80nm diameter respectively) linked by an DNA sequence. The DNA sequence combined with the OTC aptamer including its complementary sequence as well as a stem-loop structure...
A sensitive steady-state chemiluminescent aptasensor based on rolling circle amplification (RCA) was fabricated for the detection of Salmonella typhimurium. The sensor utilized aptamer modified Fe3O4 magnetic nanoparticles (MNPs) as capture probes, aptamer as recognition molecules, and Co2+ enhanced N‑(aminobutyl)-N-(ethylisoluminol) (ABEI) functional flowerlike gold nanoparticles (AuNFs) and complementary...
In this study, a gold nanoparticle-based colorimetric aptasensor for Staphylococcus aureus (S. aureus) using tyramine signal amplification (TSA) technology has been developed. First, the biotinylated aptamer specific for S. aureus was immobilized on the surface of the wells of the microtiter plate via biotin–avidin binding. Then, the target bacteria (S. aureus), biotinylated-aptamer–streptavidin-HRP...
In this work, we presented a new aptasensor for fumonisin B 1 (FB 1 ) based on fluorescence resonance energy transfer (FRET) between NaYF 4 : Yb, Ho upconversion fluorescent nanoparticles (UCNPs) and gold nanoparticles (AuNPs). The quenchers (AuNPs) were attached to the 5′ end of the molecular beacon (MB), and the donors (UCNPs) were attached to the 3′ end of the MB. In the...
A new resonance light scattering (RLS) assay of proteins is presented. In the citric acid-NaOH (pH 2.35) buffer, the RLS of Resorcinol yellow (RY)-protein system can be greatly enhanced by addition of nonionic surfactant OP, owing to the interaction between OP and RY-protein. The enhanced RLS is in proportion to the concentration of proteins in the range 0.02-4.0 μg ml -1 for both bovine...
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