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A new laccase from the filamentous fungus Podospora anserina has been isolated and identified. The 73kDa protein containing 4 coppers, truncated from its first 31 amino acids, was successfully overexpressed in Pichia pastoris and purified in one step with a yield of 48% and a specific activity of 644Umg −1 . The kinetic parameters, k cat and K M , determined at 37°C and optimal...
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