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Sunflower leaves have unidentified compounds that interfere with propidium iodide (PI) intercalation and/or fluorescence. Independently prepared pea leaf nuclei show greater PI fluorescence than nuclei from pea leaves simultaneously processed (co-chopped) with sunflower leaves. Differences in fluorescence persist after mixing the PI-stained pea and the co-chopped pea/sunflower samples, i.e. PI staining...
From observations of inbred lines ofHelianthus annuusgrown under controlled environmental conditions, we hypothesized that sunflower plants regulate DNA content in response to light quality and quantity. This hypothesis was tested under field conditions by measuring DNA content (propidium iodide fluorescence) of embryo and leaf nuclei from plants of a natural population ofHelianthus annuusL. The population...
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