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Dioscin derivatives (1–12) with a variety of substitutions at the 6′-OH of the chacotriosyl residue and the 3′,6′-anhydrosaponin derivatives (26, 30, and 32) were synthesized. All these derivatives showed much lower cytotoxicity than that of the parent dioscin, while their hemolytic activities were partially retained depending on the various 6′-O-substitutions.
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