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Capillary polystyrene-divinylbenzene (PS-DVB) monolithic columns were used to separate differentially acetylated intact IM9 protein isoforms. Compared to the unmodified form, the hydrophobic shift for intact acetylated isoforms was significant under standard reversed-phase conditions (32.5–45% acetonitrile in 10min). The high chromatographic resolution of the PS-DVB monolithic columns resulted in...
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