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We previously sequenced 8 anti-3-FL mAbs, obtained from four fusions, and found that they were all encoded by VH441, of the X24 family, and VK24B, and all mAbs had low avidity. We wanted to obtain higher avidity Abs and to ascertain if other light (L) chains could encode these Abs. We constructed a phage display library, in a modified pComb 8 vector, that contained random L chains and Fd segments...
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